Selected immunological methods

Immunological Methods

IMMUNOLOGICAL METHODS. Edited by J. F. Ackroyd. Oxford, Blackwell Scientific Publ. Co. (in U.S.A., distributed by F. A. Davis Co., Philadelphia), 1964. xvi, 628 pp. $16.00. This volume records the proceedings of a symposium organized by the Council for International Organizations of Medical Sciences (CIOMS). Although no details of time or place are given, I have been told that the meeting was h...

Journal of Immunological Methods

Five ampouled preparations of interleukin-4 (IL-41 have been evaluated by 36 laboratories in 14 countries for their suitability to serve as an international standard for this material in a joint international collaborative study for interleukin-3 (IL-3) and IL-4. The preparations were assayed in a wide range of in vitro bioassays and immunoassays. It is clear from the study that different recom...

Journal of Immunological Methods

One of the key features in the affinity maturation of antibodies is somatic hypermutation of the variable regions of immunoglobulin genes. The mutations that occur in immunoglobulin genes are detected by direct sequencing of cloned polymerase chain reaction (PCR) products. The frequencies of mutations in vivo are generally high enough to provide sufficient numbers of point mutations in order to...

Reprinted from JOURNAL OF IMMUNOLOGICAL METHODS

Bone marrow (BM)-derived dendritic cells (DC) are the most potent known antigen (Ag) presenting cell in vivo and in vitro. Detailed analysis of their properties and mechanisms of action requires an ability to produce large numbers of DC. Although DC have been isolated from several rat tissues, including BM, the yield is uniformly low. We describe a simple method for the propagation of large num...

Antipeptide Antibodies: Immunological Methods and Protocols